Fayette County is home to Lexington, Kentucky's second-largest city and the heart of the Bluegrass Region. Known for horse farms, rolling pastures, and strong land values, Fayette County offers urban amenities alongside agricultural heritage. County seat: Lexington. Nestled in Kentucky's Eastern region, Knox County, with its seat in Barbourville and a population around 31,145, offers rolling hills and access to Daniel Boone National Forest, ideal for those seeking rugged, natural beauty and opportunities for outdoor recreation on their land.